![]() Modifications of the cutoff points of the ELISA test were obtained with technical adjustments done to detect HSV-2 antibodies by ELISA and Western blot using DBS samples.ĭried blood spots ELISA Herpes simplex virus type 2 Western blot.Ĭopyright © 2018 The Authors. ELISA and Western Blot tests are both used to detect antibodies to an infection caused by the bacterium Borrelia burgdorferi (B. This laboratory utilized the same Lyme ELISA and Lyme Western blot kits (MarDx, Trinity Biotech Tray, Ireland) throughout the study period. A 1:5 dilution was used and the incubation times were increased for the Western blot.ĩ08 DBS samples were processed and the following cutoff points were determined: negative (0-3.79), undetermined (3.8-4.6) and positive (≥4.61), with sensitivity and specificity close to 95%. All serologic Lyme disease tests from the study institution were performed at a single commercial reference laboratory (personal communication, ARUP National Laboratories, Salt Lake, UT). both or either tests positive, and 3) IHA+ELISA confirmed by WB if discordant. Indeterminate results may be seen with early HIV 1/2 infections. ELISA was performed with 100μl and the Western blot with 200μl of eluted DBS. Specificity when combined with ELISA exceeds 99.9. Samples were processed by both methods to determine ELISA cutoff points using ROC curves. IgG-G2 ELISA (Human ® Diagnostics, Germany) and Western blot IgG/IgM (EUROLINE-WB, Euroimmun ® Germany) tests were modified to use DBS samples. ![]() The objective of current study was to evaluate the use of DBS samples to detect HSV-2 antibodies using commercial ELISA and Western blot tests. An antigen-antibody reaction occurs between an analyte from the tested. HSV-2 is the main cause of genital ulcers and is diagnosed mainly with serological tests. Immunohistochemistry, ELISA, and Western blotting are of the old methods that. ![]() Herpes simplex virus type 2 (HSV-2) is a sexually transmitted agent and is detected worldwide. ![]()
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